Coding

Part:BBa_K227006:Design

Designed by: WashU iGEM 2009   Group: iGEM09_Wash_U   (2009-10-12)
Revision as of 08:02, 21 October 2009 by Jrrubens (Talk | contribs) (→‎Design Notes)

puc BA coding region of R. sphaeroides


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

puc A and puc B together create the LH2 antenna necessary for Rhodobacter sphaeroides to harvest light at wavelengths 800 nm and 850 nm. A common problem in bioreactors is the uneven distribution of light between cells nearest the light source and cells farther away from the light source. One way of combating this problem is to modify the antenna size of the organism. By truncating the light-harvesting antenna, cells at the exterior of a bioreactor waste less light through Non-Photochemical Quenching, thus allowing photons to penetrate deeper and reach cells in the interior of a bioreactor. This leads to an increase in photosynthetic efficiency and productivity for the entire culture.
This construct contains the natural RBS binding sites for R. sphaeroides.
LH2 Absorption Spectra
R sphaeroides aborbance.png source: Walz et al. 1998

Source

PCR applified from plasmid pRKCBC3 provided by Dr. Neil Hunter.


References

Kiley, Patricia J.; and Kaplan, Samuel; "Cloning, DNA , and Expression of the Rhodobacter sphaeroides Light-Harvesting B800-850-a and B800-850-b Genes" Journal of Bacteriology. July 1987.[http://www.ncbi.nlm.nih.gov/pmc/articles/PMC212379/pdf/jbacter00197-0368.pdf]