Coding

Part:BBa_K5043006

Designed by: Richard Meuten   Group: iGEM24_TUDarmstadt   (2024-08-17)
Revision as of 18:07, 17 August 2024 by Richard-meuten (Talk | contribs)


pdoA2 from Mycobacterium sp. strain 6PY1

Encodes Phenanthrene ring-hydroxylating oxygenase alpha subunit. Presumably catalyzes as complex with pdoB2, phtAc and phtAd oxidation of Phenanthrene-4-carboxylate to cis-phenanthrene-3,4-dihydrodiol-4-carboxylate. This is the fifth step in pyrene biodegradation. [1]

[1] S.-J. Kim, O. Kweon, R. C. Jones, J. P. Freeman, R. D. Edmondson, and C. E. Cerniglia, "Complete and integrated pyrene degradation pathway in Mycobacterium vanbaalenii PYR-1 based on systems biology," Journal of bacteriology, vol. 189, no. 2, pp. 464–472, 2007, doi: 10.1128/jb.01310-06.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1285
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1285
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1285
    Illegal XhoI site found at 448
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1285
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1285
    Illegal NgoMIV site found at 43
    Illegal NgoMIV site found at 723
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None