Part:BBa_K5071007
BGCII-2
Sequence and Features
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 937
- 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 937
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 621
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 937
- 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 937
Illegal NgoMIV site found at 1362
Illegal NgoMIV site found at 1848
Illegal AgeI site found at 1036 - 1000COMPATIBLE WITH RFC[1000]
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Composite part BBa_K5071007 (BGCII-2)
Name: BGCII-2
Base Pairs: 2000 bp
Origin: Paceibacterales
Usage and Biology
BGCII-2 is a gene identified within a predicted terpene biosynthesis gene cluster, with bioinformatic tools suggesting a potential role in terpene production. Although its precise function remains unknown, sequence analysis indicates the presence of conserved domains common to enzymes involved in secondary metabolite pathways, highlighting its potential importance in the biosynthesis of terpenoids. Further experimental validation is required to elucidate its specific role.
Cultivation
We used PCR to amplify the BGCII-2 gene, with a length of 2000 bp. Fig 1 shows a band consistent with the target size, indicating that the target gene was successfully amplified. After agarose gel electrophoresis and gel recovery, homologous recombination was used to obtain the recombinant plasmid pETDuet-BGCII-gene792.
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