Part:BBa_K5071001
BGCI-2
Sequence and Features
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 132
Illegal PstI site found at 349
Illegal PstI site found at 439
Illegal PstI site found at 759 - 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 132
Illegal PstI site found at 349
Illegal PstI site found at 439
Illegal PstI site found at 759 - 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 132
Illegal PstI site found at 349
Illegal PstI site found at 439
Illegal PstI site found at 759 - 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 132
Illegal PstI site found at 349
Illegal PstI site found at 439
Illegal PstI site found at 759
Illegal AgeI site found at 1039 - 1000COMPATIBLE WITH RFC[1000]
Composite part BBa_K5071001 (BGCI-2)
Name: BGCI-2
Base Pairs: 1200 bp
Origin: Bacteriovoracaceae
Usage and Biology
BGCI-2 is a gene identified within a predicted terpene biosynthesis gene cluster, with bioinformatic tools suggesting a potential role in terpene production. Although its precise function remains unknown, sequence analysis indicates the presence of conserved domains common to enzymes involved in secondary metabolite pathways, highlighting its potential importance in the biosynthesis of terpenoids. Further experimental validation is required to elucidate its specific role.
Cultivation
We used PCR to amplify the BGCI-2 gene, with a length of 1200 bp. Fig 1 shows a band consistent with the target size, indicating that the target gene was successfully amplified. After agarose gel electrophoresis and gel recovery, homologous recombination was used to obtain the recombinant plasmid pETDuet-BGCI-gene123.
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