Part:BBa_K5143024

Yellow protein and BioGlue protein secreted by Saccharomyces cerevisiae

Description

This composite part corresponds to the association of two composite parts (BBa_K5143023 & BBa_K5143003) linked by a P2A system (BBa_K5143012), all under the control of a single promoter. During translation, this would give two distinct fusion proteins from the same transcriptional unit, which would then be secreted by their respective alpha factors and associate with the cellulose membrane via their CBD domains. This composite part is cloned within the backbone (BBa_K5143005), then digested by XhoI so that the composite part is framed by zones of homology. Following transformation, the whole is integrated by homologous recombination within the yeast Saccharomyces cerevisiae .

Construction

ECRIRE DES TRUCS ICI POUR LA CONSTRUCTION

References

METTRE LES REF ICI

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal EcoRI site found at 1361
  Illegal PstI site found at 2789
  Illegal PstI site found at 3092
  Illegal PstI site found at 3185
  Illegal PstI site found at 3191
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal EcoRI site found at 1361
  Illegal PstI site found at 2789
  Illegal PstI site found at 3092
  Illegal PstI site found at 3185
  Illegal PstI site found at 3191
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal EcoRI site found at 1361
  Illegal BamHI site found at 1906
  Illegal BamHI site found at 2620
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal EcoRI site found at 1361
  Illegal PstI site found at 2789
  Illegal PstI site found at 3092
  Illegal PstI site found at 3185
  Illegal PstI site found at 3191
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal EcoRI site found at 1361
  Illegal PstI site found at 2789
  Illegal PstI site found at 3092
  Illegal PstI site found at 3185
  Illegal PstI site found at 3191
  Illegal NgoMIV site found at 1876
  Illegal AgeI site found at 1915
  
• 1000
  COMPATIBLE WITH RFC[1000]