Part:BBa_K5143003

Description

Cp19k-MaSp1 is a fused protein that can be used as a bioglue. It is the result of a fusion between a spider silk protein (MaSp1) and the barnacle cement protein (Cp19k). The adhesion ability of Cp19k-MaSp1 was higher than that of individual proteins. Adhesion strength: 39.9.7 mJ/m²

Construction

The codons were optimised for synthesis and expression in Saccharomyces cerevisiae .
MaSp1 and Cp19k are fused with the GS linker: GGGGGTGGTGGTTTGGAAAGTGGAGGAGGTGGAAGT
This composite part is part of the following larger composite part: BBa_K5143024
It was synthesized in its entirety and then cloned via PCR into the following plasmid: BBa_K5143005

References

Ye L, Liu X, Li K, Li X, Zhu J, Yang S, Xu L, Yang M, Yan Y, Yan J. A bioinspired synthetic fused protein adhesive from barnacle cement and spider dragline for potential biomedical materials. Int J Biol Macromol. 2023 Dec 31;253(Pt 5):127125. doi: 10.1016/j.ijbiomac.2023.127125. Epub 2023 Sep 28. PMID: 37776922.

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal PstI site found at 746
  Illegal PstI site found at 1049
  Illegal PstI site found at 1142
  Illegal PstI site found at 1148
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal PstI site found at 746
  Illegal PstI site found at 1049
  Illegal PstI site found at 1142
  Illegal PstI site found at 1148
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BamHI site found at 577
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal PstI site found at 746
  Illegal PstI site found at 1049
  Illegal PstI site found at 1142
  Illegal PstI site found at 1148
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal PstI site found at 746
  Illegal PstI site found at 1049
  Illegal PstI site found at 1142
  Illegal PstI site found at 1148
  
• 1000
  COMPATIBLE WITH RFC[1000]