![](https://parts.igem.org/images/partbypart/icon_coding.png)
Coding
Part:BBa_K5226008:Design
Designed by: Yujiao Yang Group: iGEM24_SCUT-China-A (2024-07-28)
TD-sdaA
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal XbaI site found at 1119
Illegal SpeI site found at 646 - 12INCOMPATIBLE WITH RFC[12]Illegal SpeI site found at 646
- 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal XbaI site found at 1119
Illegal SpeI site found at 646 - 25INCOMPATIBLE WITH RFC[25]Illegal XbaI site found at 1119
Illegal SpeI site found at 646 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
Due to the presence of illegal sites in this part that do not comply with RFC 10 or Type IIS standards, synonymous mutation is required for illegal sites. Mutate the 1251bp base pair of TD-sdaA from g/c to a/t.
Source
Sequence source is from Halomonas sp. TD01 strain, found through NCBI (https://www.ncbi.nlm.nih.gov/nuccore/OV350343.1?from=2231433&to=2232800)