Part:BBa_K5143014

mRuby2 : reporter Red Fluorescent Protein

Description

mRuby 2 is a red fluorescent protein that was engineered to monitor biochemical processes in living cells using changes in Fluorescence Resonance Energy Transfer (FRET). Therefore, mRuby2 can be used as a reporter system. Here, mRuby2 was used as a transcriptional reporter, to control the transcriptional efficiency of the yeast promoter pADH1 (mettre lien part pADH1). The excitation wavelength is 559nm and the emission wavelength is 600nm.

References

1. Lam, A. et al. Improving FRET dynamic range with bright green and red fluorescent proteins. Nat Methods 9, 1005–1012 (2012). 2. Lee, M. E., DeLoache, W. C., Cervantes, B. & Dueber, J. E. A Highly Characterized Yeast Toolkit for Modular, Multipart Assembly. ACS Synth. Biol. 4, 975–986 (2015).

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]