Part:BBa_K187000

pAB is one of two universal plasmids for the BioBytes standardized in vitro assembly method. The other universal plasmid, pBA, is entered as BBa_K187001. To prepare parts inserted in pAB for assembly, primers BBa_K187365 and BBa_K187366 should be used.

The BioBytes system is based on a bead platform where constructs with long sticky ends are annealed together sequentially. This system is designed to enable the single day assembly of plasmids composed of up to about half a dozen premade parts. Complementary to existing BioBrick standards for DNA assembly, we expect this system to be useful for rapid generation of large constructs wherever an interpart spacing of 37 bp can be tolerated.

Using the plasmids pAB and pBA, as well as universal primer sequences for the PCR extraction of bricks from the plasmids, anyone will be able to use the BioBytes system for their own rapid assembly. The design ensures that the existing catalog of BioBrick parts is compatible with the system, and can be easily cloned into the universal plasmids for brick generation. We believe that this new system is ready for both use and formalization, and we have prepared a BBF RFC detailing the use of BioBytes.