Coding

Part:BBa_K4880014

Designed by: Xinran Xia   Group: iGEM23_BASIS-China   (2023-09-08)
Revision as of 15:57, 12 October 2023 by Aeolus (Talk | contribs)

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CYP736A167

This sequence encodes for the santalol synthase derived from Santalum albumand and has been codon optimized in cyanobacteria. It is a hemoprotein of the Cytochrome P450 family that converts santalene to santalol.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1228
    Illegal PstI site found at 976
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1228
    Illegal PstI site found at 976
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1228
    Illegal XhoI site found at 564
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1228
    Illegal PstI site found at 976
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1228
    Illegal PstI site found at 976
    Illegal NgoMIV site found at 65
    Illegal NgoMIV site found at 377
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 676


References

Zha W, An T, Li T, Zhu J, Gao K, Sun Z, Xu W, Lin P, Zi J. Reconstruction of the Biosynthetic Pathway of Santalols under Control of the GAL Regulatory System in Yeast. ACS Synth Biol. 2020 Feb 21;9(2):449-456.

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