Part:BBa_K4765135

ribozyme connected: Dsup + H. ex mtSSB + SAHS 33020 + XRCC1 + FEN1

Usage and Biology

This part is the combination of all the anti-desiccation and anti-UV proteins, we've transformed it to E .coli BL21 DE3 and test its performance.

Characterization

Agarose gel electrophoresis

Figure 1. Agarose gel electrophoresis of PCR products, amplified from bacterial colonies/cultures. From right lane(3) to left lane(1) indicate the successful construction of Dsup, Dsup + H. ex mtSSB, and Dsup + H. ex mtSSB + SAHS 33020.

Successful Protein Expression

Figure 2. SDS-PAGE electrophoresis of BBa K4765135 We constructed BBa K4765135 into the pET28a plasmid and transformed it into E. coli BL21 DE3. This lane represents the leaky expression of BBa K4765135. As indicated by the red arrow, we successfully expressed Rv Dsup, H. ex mtSSB, SAHS 33020 and XRCC1 in this part. Sequence and Features Assembly Compatibility: 10 COMPATIBLE WITH RFC[10] 12 INCOMPATIBLE WITH RFC[12] Illegal NheI site found at 3307 21 INCOMPATIBLE WITH RFC[21] Illegal BamHI site found at 5485 23 COMPATIBLE WITH RFC[23] 25 INCOMPATIBLE WITH RFC[25] Illegal NgoMIV site found at 1516 Illegal NgoMIV site found at 6063 Illegal AgeI site found at 230 Illegal AgeI site found at 301 Illegal AgeI site found at 5169 1000 INCOMPATIBLE WITH RFC[1000] Illegal BsaI site found at 2149 Illegal BsaI.rc site found at 227 Illegal BsaI.rc site found at 1784 Illegal BsaI.rc site found at 1865 Illegal BsaI.rc site found at 4357 Illegal SapI site found at 1678 Illegal SapI.rc site found at 956