Composite

Part:BBa_K225001

Designed by: Nasa SAVORY   Group: iGEM09_Tokyo-Nokogen   (2009-10-15)
Revision as of 15:44, 5 May 2014 by Drew (Talk | contribs) (→‎Expecting function (without experimental proof))

Green light activated actuator

Green light activated actuator composed of the phycocyanobilin synthesis genes (ho1 and pcyA) and the green light receptor (GlrN).

Usage and Biology

GlrN is the fusion protein of light responsive domains of CcaS and EnvZ designed by changing the histidine kinase region of CcaS to EnvZ domain of cph8 (BBa_I15010).

  • Requires phycocyanobilin biosynthetic genes (BBa_I15008 and BBa_I15009) for chromophore formation.
  • Must be used in E. coli deficient in natural EnvZ.

Expected function (without experimental proof)

Exposure to green light (535 nm) phosphorylate EnvZ histidin kinase domain. The activated EnvZ phosphorylates endogenous OmpR, a transcription factor which activates transcription from the OmpR positive promoter (BBa_R0083).

NOTE. This part may be thought of as a sensor for green light, not an actuator [D.Endy, 5 May 2014]

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 2202
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1564
    Illegal BglII site found at 2456
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1117
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None