Composite

Part:BBa_K4645022:Design

Designed by: Yuanpu Zhang   Group: iGEM23_HZAU-China   (2023-10-12)
Revision as of 12:33, 12 October 2023 by Zhangyuanpu (Talk | contribs) (→‎References)

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HepT-MntA toxin-antitoxin--CI-PR System


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 856
    Illegal NheI site found at 1561
    Illegal NheI site found at 1584
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We constructed a circuit to validate the function of the toxin-antitoxin system (HepT/MntA) and the CI-PR system.We constructed the CI protein downstream of the LacI promoter, and constitutively expressed HepT, and connected the antitoxin protein MntA downstream of a promoter repressed by the CI protein, using the pet28a plasmid transformed into E. coli BL21(DE3).