Primer

Part:BBa_K4712063

Designed by: Ke Zhang   Group: iGEM23_SMS-Shenzhen   (2023-09-29)
Revision as of 11:42, 12 October 2023 by Ke-666 (Talk | contribs)


NME-R4

The primers were designed using NCBI BLAST and SanpGene to achieve efficient and specific amplification. This primer in RPA serves as the initial binding point for the amplification process, ensuring the specificity of the reaction by targeting the desired Neisseria meningitidis DNA or RNA sequences. The primers provided data for mathematical modeling for further primer design.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Protocol: 1. For a 20μL reaction system:

ReagentStock ConcentrationVolume Added（μL）
Forward Primer10μM1
Reverse Primer10μM1
Rehydration Buffer (2X)10
DNA Template10nM/L2
ddH2OTo 18
Starter (10X)2

2. Gently tap to mix several times, briefly centrifuge, repeat 3 times (mix gently to avoid vigorous vortexing). 3. Incubate at 37°C for 20 minutes. 4. After heating at 65°C for 10 minutes, proceed to gel electrophoresis.

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Parameters
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