Coding

Part:BBa_K4586032

Designed by: Mohamed Mohamed Saad Aboelghare   Group: iGEM23_AFCM-Egypt   (2023-10-05)
Revision as of 11:38, 12 October 2023 by E m1 (Talk | contribs) (→‎Experimental Characterization)


Anti CD19

Usage and Description

This part codes for the light and heavy chain scFv of anti CD19, that represent the extra cellular domain in syn notch receptor sensitive for CD19.

This figure show our Syn-Notch receptor which represent Anti-CD19 as our ligand binding domain (LBD).

Experimental Characterization

In order to amplify this DNA part, we used PCR amplification to reach the desired concentration to complete our experiments using specific forward and reverse primers, running the parts on gel electrophoresis as this part presents lane (p5) including lamb2p and anti-CD19 , and (p1) including (cd8-alpha,histag,zf21..16/vp64 ,and anti-CD19),and then we measured the specific concentration of the running part using Real-Time PCR as shown in the following figure.





We performed the double digestion method for this part in the prefix and suffix with its specific restriction enzyme and applied this part to gel electrophoresis as shown in the following figure lane(P1) and lane(p5).


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 444


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