Part:BBa_K4990011
Bacteria-Targeting Rod
Usage in short
You can use it to achieve BL-Fn adhesion.
You need to know
Acurate targeting is one important characteristics for many emerging cancer therapies, that is also what ourproject’s ambition. We designed two fusion proteins, they function like fishingrod, so we gave them the name: Fishing Rod Proteins (FRPs). The one that targetFn names Bacteria Tageting Rod(BTR), the other that target CRC cells names Tumor Tageting Rod(TTR). FRPs are displayed on engineered BL membranes, helping BLtarget to the correct place. Of course, their targets are cancer cells and Fn,rather than fish. Each FRP consists of four parts (Figure1): the signal peptide(white),helping FRPs locate on cell membrane; the membrane protein(pink), the base ofFRPs; and the linker(blue), helping FRPs become flexible; and the targeting fragments(yellow), the most pivotal role in FRPs
What is it
It consists of the GL-BP membrane protein, three repeated G4S linker peptides, and HlpA. Essentially, it involves the surface display technique to express HlpA on the BL membrane for adhesion to HSPG, thereby achieving adhesion between BL and CRC.
Sequence and Features
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 358
Illegal PstI site found at 1450 - 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 358
Illegal PstI site found at 1450 - 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 771
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 358
Illegal PstI site found at 1450 - 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 358
Illegal PstI site found at 1450
Illegal NgoMIV site found at 1050 - 1000COMPATIBLE WITH RFC[1000]
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