Coding

Part:BBa_K4825050:Design

Designed by: Ni Weizhao   Group: iGEM23_GreatBay-SCIE   (2023-10-11)
Revision as of 12:23, 12 October 2023 by Aisy (Talk | contribs)

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CDC9-LEU1-COX6-LEU2-COX4-LEU4^S547∆


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 625
    Illegal EcoRI site found at 3427
    Illegal EcoRI site found at 4534
    Illegal EcoRI site found at 5323
    Illegal EcoRI site found at 7400
    Illegal EcoRI site found at 7864
    Illegal XbaI site found at 61
    Illegal XbaI site found at 1324
    Illegal XbaI site found at 1438
    Illegal XbaI site found at 7641
    Illegal SpeI site found at 1479
    Illegal PstI site found at 4508
    Illegal PstI site found at 5003
    Illegal PstI site found at 5288
    Illegal PstI site found at 8330
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 625
    Illegal EcoRI site found at 3427
    Illegal EcoRI site found at 4534
    Illegal EcoRI site found at 5323
    Illegal EcoRI site found at 7400
    Illegal EcoRI site found at 7864
    Illegal NheI site found at 1002
    Illegal SpeI site found at 1479
    Illegal PstI site found at 4508
    Illegal PstI site found at 5003
    Illegal PstI site found at 5288
    Illegal PstI site found at 8330
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 625
    Illegal EcoRI site found at 3427
    Illegal EcoRI site found at 4534
    Illegal EcoRI site found at 5323
    Illegal EcoRI site found at 7400
    Illegal EcoRI site found at 7864
    Illegal BglII site found at 2209
    Illegal BamHI site found at 1155
    Illegal BamHI site found at 2121
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 625
    Illegal EcoRI site found at 3427
    Illegal EcoRI site found at 4534
    Illegal EcoRI site found at 5323
    Illegal EcoRI site found at 7400
    Illegal EcoRI site found at 7864
    Illegal XbaI site found at 61
    Illegal XbaI site found at 1324
    Illegal XbaI site found at 1438
    Illegal XbaI site found at 7641
    Illegal SpeI site found at 1479
    Illegal PstI site found at 4508
    Illegal PstI site found at 5003
    Illegal PstI site found at 5288
    Illegal PstI site found at 8330
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 625
    Illegal EcoRI site found at 3427
    Illegal EcoRI site found at 4534
    Illegal EcoRI site found at 5323
    Illegal EcoRI site found at 7400
    Illegal EcoRI site found at 7864
    Illegal XbaI site found at 61
    Illegal XbaI site found at 1324
    Illegal XbaI site found at 1438
    Illegal XbaI site found at 7641
    Illegal SpeI site found at 1479
    Illegal PstI site found at 4508
    Illegal PstI site found at 5003
    Illegal PstI site found at 5288
    Illegal PstI site found at 8330
    Illegal NgoMIV site found at 4051
    Illegal AgeI site found at 3040
    Illegal AgeI site found at 6253
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 911
    Illegal SapI site found at 5513
    Illegal SapI.rc site found at 895


Design Notes

The introduction of signal peptides which are originally CDC9, COX6 and COX4 results in targeting LEU1, LEU2 and LEU4 to mitochondrion, which would increase the regional enzyme concentration for enhancement in the production of KIC.


Source

Saccharomyces cerevisiae

References

1. Sarah K. Hammer, Yanfei Zhang, and José L. Avalos

2. ACS Synthetic Biology 2020 9 (3), 546-555

3. DOI: 10.1021/acssynbio.9b00420

4. Willer, M., Rainey, M., Pullen, T., and Stirling, C. J. (1999) The yeast CDC9 gene encodes both a nuclear and a mitochondrial form of DNA ligase I. Curr. Biol. 9, 1085−1094.

5. "ACS Synth. Biol. 2020, 9, 3, 546–555 

Publication Date:February 12, 2020

6. https://doi.org/10.1021/acssynbio.9b00420"