Part:BBa_K4895202
mScarlet Optogentically controlled plasmid backbone (N-terminally His tagged)
Often, teams may seek to test multiple enzymes independently to compare their activity. To simplify the process of creating several expression vectors we used a optogentically controlled plasmid backbone wherein the red fluorescent protein mScarlet would act as our “spacer” sequence. mScarrelt dually acts as a visible selection marker to confirm that cloning was successful or not. The mScarlet ”spacer” sequence also contains a promoter, terminator, and ribosome binding site to ensure bacterial expression.There are Bbs1 cut sites flanking the Scarlet spacer region. In combination with the accompanying golden gate template (BBa0009123) teams can easily use this construct and template to assemble multiple things in only one pcr. Successful transformants are expected to appear white.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 1103
Illegal NgoMIV site found at 1235
Illegal NgoMIV site found at 1329
Illegal NgoMIV site found at 1622
Illegal NgoMIV site found at 2116
Illegal NgoMIV site found at 2134
Illegal NgoMIV site found at 2224
Illegal AgeI site found at 1454
Illegal AgeI site found at 2582
Illegal AgeI site found at 4451 - 1000COMPATIBLE WITH RFC[1000]
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