Composite

Part:BBa_K4591012

Designed by: XinTong Shen   Group: iGEM23_ZJUT-China   (2023-10-08)
Revision as of 08:27, 12 October 2023 by Kaga (Talk | contribs)


P43-DepoPETase-MHETase-T500

Usage and Biology

This circuit contains the coding sequences of P43, DEPO-PETase, and MHETase. The expression of enzyme genes related to this route is controlled by the frequently expressed promoter P43, which degrades PET into TPA and achieves the preliminary degradation of PET.

Basic components of the circuit

P43：Promoter 43 is a constitutive promoter that constitutively expresses the P43 protein in B.subtilis.

DEPO：DepoPETase is the best mutant obtained by researchers using a novel high-throughput screening method based on fluorescence detection for directed evolution of IsPETase.

MHETase：MHETase is discovered from Ideonella sakaiensis 201-F6, it is a enzyme which can hydrolyze MHET, which is the main product of PETase when hydrolyzing PET.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 421
    Illegal PstI site found at 588
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 421
    Illegal PstI site found at 588
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 421
    Illegal XhoI site found at 505
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 421
    Illegal PstI site found at 588
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 421
    Illegal PstI site found at 588
    Illegal NgoMIV site found at 275
    Illegal NgoMIV site found at 2010
    Illegal AgeI site found at 1230
    Illegal AgeI site found at 1347
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None