RNA

Part:BBa_K4683017:Experience

Designed by: Sahana Narayanan, Kate Sharer   Group: iGEM23_Lambert-GA   (2023-10-12)
Revision as of 06:04, 12 October 2023 by Natalietan (Talk | contribs) (→‎Applications of BBa_K4683017)


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Applications of BBa_K4683017


Gel Experimentation
We performed RCA on these six miRNAs (miR-20b, miR-328,miR-146, miR-150, miR-122, miR-30c) and ran the subsequent rolling circle product (RCP) on a 1% agarose gel (see Fig.). The gel exhibited a fluorescent band of DNA very close to the well, indicating that a long strand of DNA greater than 1 kB - our RCP - was produced. Therefore, we can validate that our reaction was successful.
Figure 1. Image of gel ran with miRNA-1 RCP product; A: eRCA with 40.8 pM miR-1; B: negative control (no enzymes)
Figure. RCP from RCA reaction with miR-20b, miR-328,miR-146, miR-150, miR-122, and miR-30c; visible bands can be seen near the top of the wells.

User Reviews

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