DNA
PQQGDH

Part:BBa_K4893001

Designed by: Corinne Drabenstott   Group: iGEM23_UNC-ChapelHill   (2023-10-07)
Revision as of 05:50, 12 October 2023 by Cndraben (Talk | contribs)

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Pyrroloquinoline Quinone Glucose Dehydrogenase

A.calcoaceticus derived PQQGDH is a dimeric enzyme which is recombinantly produced using E. coli. It is responsible for oxidizing glucose into glucono-δ-lactone which is then hydrolyzed into gluconic acid. In PQQGDH holo form, it possesses the PQQ cofactor that gives the protein its electron-carrying abilities. This enzyme has been most widely used for glucose-sensing applications. In our project, we present a new application of this enzyme in pyocyanin sensing for P. aeruginosa detection.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 450
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

SDS page of Crude Sample of PQQGDH bba-k4893001-sds-page.jpg

Modeling of PQQGDH in PyMOL bba-k4893001-model.jpg


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Categories
Parameters
None