DNA
PQQGDH

Part:BBa_K4893001

Designed by: Corinne Drabenstott   Group: iGEM23_UNC-ChapelHill   (2023-10-07)
Revision as of 05:28, 12 October 2023 by Cndraben (Talk | contribs)


Pyrroloquinoline Quinone Glucose Dehydrogenase

A.calcoaceticus derived PQQGDH is a dimeric enzyme which is recombinantly produced using E. coli as a host microorganism. It is responsible for oxidizing glucose into glucono-δ-lactone which is then hydrolyzed into gluconic acid. In PQQGDH holo form, it possesses the PQQ cofactor that gives its redox abilities. This enzyme has been most widely used for glucose-sensing applications. In our project, we present a new application of this enzyme in pyocyanin sensing for P. aeruginosa detection.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 450
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

bba-k4893001-sds-page.jpg bba-k4893001-model.jpg


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Categories
Parameters
None