Composite

Part:BBa_K208041

Designed by: USU iGEM 2009   Group: iGEM09_Utah_State   (2009-10-20)
Revision as of 01:18, 23 October 2009 by Liblint (Talk | contribs)

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Lac Promoter/RBS/PelB/PhaP/Terminator
Phasin protein with a fused PelB signal peptide, promoter, rbs, and terminator. Please consult the individual pages for these parts for more information.

NOTE: The scar between the signal peptide and the protein is not presented correctly in the sequence below. The scar is the Silver-fusion scar (ACTAGA), which allows for in-frame fusion. Additionally, the scars before the signal peptide and after the protein are affected by these parts each having the Silver-fusion prefix and suffix.

Usage and Biology

This part is for the secretion of phasin via the Sec secretion mechanism.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 280
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None