Coding

Part:BBa_K4830032:Design

Designed by: Beibitkyzy Aruzhan   Group: iGEM23_NTU-Singapore   (2023-10-11)
Revision as of 03:22, 12 October 2023 by Aruzhan001 (Talk | contribs)

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CaMV RT


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

No alterations have been made to the original sequence. In order to use it for prime editing, this RT was fused to nickase Cas9 and combined with CMV promoter and plasmid backbone.

Source

NCBI ACCESSION: NP_056728

References

Franck, A. et al. (1980) ‘Nucleotide sequence of cauliflower mosaic virus DNA’, Cell, 21(1), pp. 285–294. doi:10.1016/0092-8674(80)90136-1.