Composite

Part:BBa_K4619012

Designed by: Yuqi Liu   Group: iGEM23_UCAS-China   (2023-10-11)
Revision as of 03:30, 12 October 2023 by BillyLii (Talk | contribs)

QS-LuxRm-PluxR

Full IY-tRNA synthase and amber suppressor tRNA sequence. This part codes for IY( 3-iodo-L-tyrosine) -tRNA synthase, and transcribes into Amber suppressor tRNA, works together with the IY(3-iodo-L-tyrosine)-tRNA synthase to incorporate unnatural amino acid 3-iodo-L-tyrosine in an amber-specific means.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 789
    Illegal NheI site found at 812
    Illegal NheI site found at 895
    Illegal NheI site found at 918
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 412
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 823
    Illegal SapI.rc site found at 962


Functional Parameters

[edit]
Categories
Parameters
None