Plasmid

Part:BBa_K4815011:Experience

Designed by: Chen Xi   Group: iGEM23_NJU-China   (2023-10-11)
Revision as of 03:33, 12 October 2023 by ChenXi (Talk | contribs) (→‎Applications of BBa_K4815011)


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Applications of BBa_K4815011

We used a synthetic promoter to drive the expression of the YeGFP gene on the same plasmid, while the TEF1 promoter was used in the reverse orientation to drive the expression of the mCherry gene. Additionally, we incorporated a lactose-inducible switch to enhance safety. We utilized flow cytometry to monitor the two fluorescence signals excited by different light channels and analyzed the corresponding data. We plotted the natural logarithm of the ratio of GFP to mCherry (ln(GFP/mCherry)) as a frequency distribution graph to showcase the relative expression strength of different promoters in yeast. The figure bellow shows success in utilizing the plasmids in detecting fluorescence intensity.

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