Intermediate

Part:BBa_K208022:Design

Designed by: USU iGEM 2009   Group: iGEM09_Utah_State   (2009-10-20)
Revision as of 22:16, 21 October 2009 by Ahatch (Talk | contribs) (→‎Design Notes)

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GeneIII Signal Peptide/GFP/Double Terminator


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 64
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 583
    Illegal XhoI site found at 484
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

GFP gene with secretion tag. No promoter or RBS. Silver fusion compatible. In pSB1AK3. Silver Fusion

Source

Composite

References