Regulatory

Part:BBa_K4788004:Design

Designed by: Zhaocheng Li   Group: iGEM23_LZU-CHINA   (2023-10-09)
Revision as of 01:56, 12 October 2023 by Zcli lzu (Talk | contribs) (→‎Design Notes)

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FadO operator


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

It is a basic operator component that can combine with the FadR gene and FadBA promoter gene to form oleic acid inducers, exerting rich functions. And changing its position and quantity can change the induction threshold of the oleic acid inducer.

Source

We synthesize this gene.

References

Ma Y, Zheng X, Lin Y, Zhang L, Yuan Y, Wang H, Winterburn J, Wu F, Wu Q, Ye JW, Chen GQ. Engineering an oleic acid-induced system for Halomonas, E. coli and Pseudomonas. Metab Eng. 2022 Jul;72:325-336. doi: 10.1016/j.ymben.2022.04.003. Epub 2022 May 2. PMID: 35513297.