Translational_Unit

Part:BBa_K4855003:Design

Designed by: Slavil Peykov   Group: iGEM23_Bulgaria   (2023-10-11)
Revision as of 23:23, 11 October 2023 by Slavil (Talk | contribs) (→‎Design Notes)

(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)


J23101 - T7 - medium RBS - blaNDM2&5 CDS


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Assembled following the rules of the BioBrick RFC10 assembly procedure from parts J23101 and BBa_K4855002. Dual promoters: J23101 for screening and T7 for mutagenesis.

Source

This part was designed to be cloned in low copy number vectors and used with the dual gene-specific mutator system from Addgene (doi: 10.1093/nar/gkad266)

References