Composite
Part:BBa_K4757062
Designed by: Marik Müller Group: iGEM23_Heidelberg (2023-10-11)
TPA sensing XylS-K38R-L224Q/Pm expressing mKate2
Terephthalic acid sensing transcription factor - fluorescence mKate2 expression cassette..... to be continued...
Engnineering journey
Mutation -> weak expression/no expression after mutation -> enhancing with RBS -> still weak/no expression -> co-induction -> significant changes could be detected
co-induction showed so low exprssion -> pEM7 promoter was used to substitute Ps1/Ps2 -> high expression but leaky
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 212
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 934
- 1000COMPATIBLE WITH RFC[1000]
[edit]
Categories
Parameters
None |