Coding

Part:BBa_K4825046:Design

Designed by: Ni Weizhao   Group: iGEM23_GreatBay-SCIE   (2023-10-11)
Revision as of 09:00, 12 October 2023 by NiWeizhao (Talk | contribs)


ARO10-ADH7-ATF1


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1791
    Illegal EcoRI site found at 3418
    Illegal SpeI site found at 742
    Illegal PstI site found at 620
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1791
    Illegal EcoRI site found at 3418
    Illegal NheI site found at 2385
    Illegal SpeI site found at 742
    Illegal PstI site found at 620
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1791
    Illegal EcoRI site found at 3418
    Illegal BglII site found at 3105
    Illegal BamHI site found at 2146
    Illegal BamHI site found at 4432
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1791
    Illegal EcoRI site found at 3418
    Illegal SpeI site found at 742
    Illegal PstI site found at 620
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1791
    Illegal EcoRI site found at 3418
    Illegal SpeI site found at 742
    Illegal PstI site found at 620
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 3532
    Illegal SapI.rc site found at 4540


Design Notes

By overexpression of ARO10 and ADH7, the supply of precursors isopenaldehyde and isopentanol is increased, and the overexpression of ATF1 directly enhances the corresponding conversion to isoamyl acetate. Therefore, The composite part increases the production of isoamyl acetate in S. cerevisiae.


Source

Saccharomyces cerevisiae

References

"1. Hammer SK, Zhang Y, Avalos JL. Mitochondrial Compartmentalization Confers Specificity to the 2-Ketoacid Recursive Pathway: Increasing Isopentanol Production in Saccharomyces cerevisiae. ACS Synth Biol. 2020 Mar 20;9(3):546-555. doi: 10.1021/acssynbio.9b00420. Epub 2020 Feb 21. PMID: 32049515. 2. Yuan J, Chen X, Mishra P, Ching CB. Metabolically engineered Saccharomyces cerevisiae for enhanced isoamyl alcohol production. Appl Microbiol Biotechnol. 2017 Jan;101(1):465-474. doi: 10.1007/s00253-016-7970-1. Epub 2016 Nov 15. PMID: 27847988."

Verstrepen KJ, Van Laere SD, Vanderhaegen BM, Derdelinckx G, Dufour JP, Pretorius IS, Winderickx J, Thevelein JM, Delvaux FR. Expression levels of the yeast alcohol acetyltransferase genes ATF1, Lg-ATF1, and ATF2 control the formation of a broad range of volatile esters. Appl Environ Microbiol. 2003 Sep;69(9):5228-37. doi: 10.1128/AEM.69.9.5228-5237.2003. PMID: 12957907; PMCID: PMC194970.