Coding

Part:BBa_K4825046

Designed by: Ni Weizhao   Group: iGEM23_GreatBay-SCIE   (2023-10-11)
Revision as of 12:18, 12 October 2023 by Aisy (Talk | contribs)

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ARO10-ADH7-ATF1

This composite part contains the part ARO10 BBa_K4825015, ADH7 BBa_K4825016 and ATF1 BBa_K4825017. In the pathway of producing isoamyl acetate in S. cerevisiae, ARO10 encodes for 2-oxoacid decarboxylase, which is reponsible for converting 2-ketoisocaproate (KIC) to isopentaldehyde. ADH7, which encodes for alcohol dehydrogenase 7, is responsible for converting isopentaldehyde to isopantanol. ATF1 encodes for alcohol acetyltransferase, which catalyzes the conversion of isopenanol to isopentyl acetate (isoamyl acetate).

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1791
    Illegal EcoRI site found at 3418
    Illegal SpeI site found at 742
    Illegal PstI site found at 620
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1791
    Illegal EcoRI site found at 3418
    Illegal NheI site found at 2385
    Illegal SpeI site found at 742
    Illegal PstI site found at 620
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1791
    Illegal EcoRI site found at 3418
    Illegal BglII site found at 3105
    Illegal BamHI site found at 2146
    Illegal BamHI site found at 4432
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1791
    Illegal EcoRI site found at 3418
    Illegal SpeI site found at 742
    Illegal PstI site found at 620
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1791
    Illegal EcoRI site found at 3418
    Illegal SpeI site found at 742
    Illegal PstI site found at 620
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 3532
    Illegal SapI.rc site found at 4540


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