Regulatory

Part:BBa_K4788002:Design

Designed by: Zheng Huang   Group: iGEM23_LZU-CHINA   (2023-10-05)
Revision as of 21:54, 11 October 2023 by Zcli lzu (Talk | contribs) (→‎References)

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Oleic acid inducer


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 764
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The front of this part requires a promoter to express the FadR protein in order to achieve oleic acid response function. Therefore, in practical use, appropriate promoters or plasmid skeletons should be selected. In longer-term applications, we can try to change the number and position of FadO operators to change the oleic acid induction threshold to achieve more precise promoter regulation.

Source

We synthesized it

References

Ma Y, Zheng X, Lin Y, Zhang L, Yuan Y, Wang H, Winterburn J, Wu F, Wu Q, Ye JW, Chen GQ. Engineering an oleic acid-induced system for Halomonas, E. coli and Pseudomonas. Metab Eng. 2022 Jul;72:325-336. doi: 10.1016/j.ymben.2022.04.003. Epub 2022 May 2. PMID: 35513297.