Coding

Part:BBa_K4825025:Design

Designed by: Cai Luxi   Group: iGEM23_GreatBay-SCIE   (2023-10-11)
Revision as of 08:33, 12 October 2023 by NiWeizhao (Talk | contribs)


AraC-NLS


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

LEU2 is used as the yeast auxotrophic marker in this part, instead of normally URA3, which allows the co-transformation of the plasmid of this part and the plasmid of arabinose promoters.


Source

Escherichia coli

References

"Robert Schleif. Regulation of the l-arabinose operon of Escherichia coli. Trends in Genetics, Volume 16, Issue 12, 2000, Pages 559-565, ISSN 0168-9525, https://doi.org/10.1016/S0168-9525(00)02153-3.

Schleif R. A Career's Work, the l-Arabinose Operon: How It Functions and How We Learned It. EcoSal Plus. 2022 Dec 15;10(1):eESP00122021. doi: 10.1128/ecosalplus.ESP-0012-2021. Epub 2021 Aug 18. PMID: 36519894."