Protein_Domain

Part:BBa_K203108

Designed by: Michael Bartoschek   Group: iGEM09_Heidelberg   (2009-10-15)
Revision as of 09:24, 20 October 2009 by Larsplus (Talk | contribs) (→‎Functional Parameters)

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Plasma membrane targeting (Myrpalm)


Localization sequence to direct a protein into the cell membrane. This part has the BBb-standard restriction sites.



Usage and Biology

This localization signal is located at the N-terminal end of the amino acid chain. The myrpalm signalsequence causes a myristoylation and palmitoylation of the targeted protein. Both modifications lead to a binding to the cell membrane.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 86
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 49
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 46


Functional Parameters

n/aPlasma membrane targeting (Myrpalm)

Shown to localize proteins to the plasma membrane. An expression device consisting of Part:BBa_I712004 (CMV) Part:BBa_K203103 (GFP), Part:BBa_K203108 and Part:BBa_K203102 (terminator) was assembled for this characterization.

Myrpalm.jpg

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Categories
Parameters
n/aPlasma membrane targeting (Myrpalm)