RNA

Part:BBa_K4830008:Design

Designed by: Beibitkyzy Aruzhan   Group: iGEM23_NTU-Singapore   (2023-10-10)
Revision as of 20:54, 11 October 2023 by Aruzhan001 (Talk | contribs) (→‎Design Notes)


POAG snp1-3 ngRNA


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The design of pegRNA includes the U6 promoter, spacer, guide RNA scaffold, rttpbs and polyA tail. The unique pegRNA was designed based on SNP data.

Source

SNPs were retrieved from the paper cited in References.

References

Gharahkhani, P. et al. (2021) ‘Genome-wide meta-analysis identifies 127 open-angle glaucoma loci with consistent effect across ancestries’, Nature Communications, 12(1). doi:10.1038/s41467-020-20851-4.