Reporter
Part:BBa_K176011
Designed by: Chao Li, Danqian Liu, Hao Jiang Group: iGEM09_USTC (2009-06-30)
pCon 1.00(J23100)->RBS+GFP+T
pCon 1.00(J23100)+I13504
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 705
MEASUREMENT
DATA
COLONY NUMBER | T(min) | OD600 | FLU(arbitrary) | ST.FLU(ustc_st.1) | FLU/OD600(arbitrary) | ST.FLU/OD600(ustc_st.1) |
Ⅰ | 0 | 0.071 | 132.07 | 527.9210137 | 1860.140845 | 7435.507235 |
Ⅰ | 30 | 0.076 | 144.49 | 577.5672543 | 1901.184211 | 7599.569135 |
Ⅰ | 60 | 0.101 | 157.6633333 | 630.2247805 | 1561.023102 | 6239.849312 |
Ⅰ | 90 | 0.093 | 160.8866667 | 643.1093523 | 1729.964158 | 6915.154326 |
Ⅰ | 120 | 0.116 | 176.33 | 704.8407083 | 1520.086207 | 6076.213003 |
Ⅰ | 150 | 0.122666667 | 185.0633333 | 739.7503031 | 1508.668478 | 6030.573123 |
Ⅰ | 180 | 0.153 | 197.8966667 | 791.0487535 | 1293.442266 | 5170.253291 |
Ⅱ | 0 | 0.07 | 144.1 | 576.0083143 | 2058.571429 | 8228.690205 |
Ⅱ | 30 | 0.075333333 | 136.1066667 | 544.0567081 | 1806.725664 | 7221.991701 |
Ⅱ | 60 | 0.084333333 | 144.92 | 579.2860855 | 1718.418972 | 6869.004966 |
Ⅱ | 90 | 0.092 | 157.4133333 | 629.22546 | 1711.014493 | 6839.407174 |
Ⅱ | 120 | 0.098 | 164.73 | 658.4722389 | 1680.918367 | 6719.104478 |
Ⅱ | 150 | 0.117 | 179.78 | 718.6313307 | 1536.581197 | 6142.148126 |
Ⅱ | 180 | 0.135666667 | 194.6566667 | 778.0975603 | 1434.815725 | 5735.362853 |
For the details of the definition of ST.FLU and the unit ustc_st1, please click here.
PLOT
[edit]
Categories
Parameters
n/a | pCon 1.00(J23100)->RBS+GFP+T |