Part:BBa_K4586028
CCP1 exosomes receptor
Usage and Description
This composite parts codes for lamp2b which is a transmembrane protein known as lysosome-associated membrane glycoprotein 2b, which is unique to the membrane of exosomes and its external domain conjugated to CCP1 to mediate its expression on the surface of the exosomes.
Figure 1. illustrates the structure of CCP1 within the extracellular domain of the Syn notch receptor expressed on the surface of the engineered MSC
Figure 2: This figure illustrates the construction of our engineered exosomes that express citrullinated vimentin on their membranes conjugated to a transmembrane protein known as lysosome-associated membrane glycoprotein 2b (lamp2b), which is unique to the membrane of exosomes.
literature characterization of ccp1
The study used gel electrophoresis to structurally characterize CCP1 compared to other parts. In Addition to studying its presence and association with neural tissues in different cross sections.
A)They observed ccp1 in proteins of brain, spinal cord and peripheral nerves.They confirmed that by detection of extra band in HEK293 transfected with ccp1-YFP fusion proteins and absence of signals in pcd mouse brain B)They found relation of ccp1 with myelinated axons(NF200) but not compact myelin (MBP) C)They found ccp1 in motor neurons( CHAT) in the ventral horn of spinal cord .
Literature Characterization of lamp2B
The study used X-Gal staining of brain sections from double transgenic mice to show the effect of active Notch1 signaling in mice. The results suggest that the activation of the N1-Gal4VP16 reporter is consistent with the activity of the endogenous Notch1 receptor. This reporter mouse is a powerful tool for visualizing active Notch1 signaling in embryonic and post-natal development in vivo.
To confirm that iRGD-Lamp2b was successfully transfected into the imDCs, the amounts of iRGD-Lamp2b mRNA was measured 36 hours after transfection using RT-PCR.The results showed that the transfected imDCs expressed high levels of iRGD-Lamp2b mRNA, compared to the untransfected imDCs. This confirmed that the transfected imDCs were producing iRGD-positive exosomes.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NotI site found at 670
Illegal NotI site found at 679 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 614
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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