Coding
vqmA

Part:BBa_K4645004

Designed by: Yutong Chen   Group: iGEM23_HZAU-China   (2023-10-11)
Revision as of 15:02, 11 October 2023 by Lloheha (Talk | contribs)


VqmAphage

This part is meant to express vqmA gene which would translate into protein vqmAphage.Vibrio cholerae uses a quorum-sensing (QS) system composed of the autoinducer3,5-dimethylpyrazin-2-ol (DPO) and receptor VqmA, which together repress genes for virulence and biofilm formation. VqmAVc activates expression of the vqmR gene encoding the small regulatory RNA VqmR. Vibriophage VP882 encodes a VqmA homolog called VqmAPhage that activates the qtip promoter (Pqtip). It’s worth noting that, VqmAPhage can activate vqmR expression but VqmAVc cannot activate expression of qtip. Promoter selectivity is driven by each VqmA DNA-binding domain and key DNA sequences in the vqmR and qtip promoters are required to maintain specificity. Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 535
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None