Coding

Part:BBa_K4623004:Design

Designed by: Yixin Huang   Group: iGEM23_BNU-China   (2023-10-08)
Revision as of 12:57, 11 October 2023 by Dooooog (Talk | contribs) (→‎References)

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Basic Silinker (TrxA-His-thrombin-mSA-SBP)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 445
    Illegal AgeI site found at 505
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The sequence is appended with an His tag, allowing for purification using a nickel column.


Source

It is a new fusion protein we have designed. We combined the sequence of Trx-His-thrombin-mSA-SBP.

References

[1]Sano, T., & Cantor, C. R. (1990). Expression of a cloned streptavidin gene in Escherichia coli. Proceedings of the National Academy of Sciences of the United States of America, 87 (1), 142–146.

[2]Lim, K. H., Huang, H., Pralle, A., & Park, S. (2011). Engineered streptavidin monomer and dimer with improved stability and function. Biochemistry, 50(40), 8682–8691.

[3] Demonte, D., Dundas, C. M., & Park, S. (2014). Expression and purification of soluble monomeric streptavidin in Escherichia coli. Applied microbiology and biotechnology, 98 (14), 6285–6295.