Coding
Part:BBa_K4830017:Design
Designed by: Beibitkyzy Aruzhan Group: iGEM23_NTU-Singapore (2023-10-11)
Ll.ltrA RT
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The original sequence of the protein was not altered in anyway. In order to use it for prime editing, this part has been fused to nickase Cas9 and combined with CMV promoter and plasmid backbone.
Source
NCBI n: