Regulatory

Part:BBa_K264000:Design

Designed by: Adam Bower   Group: iGEM09_Virginia_Commonwealth   (2009-10-19)
Revision as of 01:54, 20 October 2009 by Bowerag (Talk | contribs) (→‎References)

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Modular UP-element Design 1


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

A spacer, CT, is placed between the UP element and subsequent promoters to maintain proper alignment with RNAP.


Source

Estrem ST, Gaal T, Ross W, Gourse RL, Identification of an UP element consensus sequence for bacterial promoters, PNAS, (1998), 95, 9761-9766.

References

Estrem ST, Gaal T, Ross W, Gourse RL, Identification of an UP element consensus sequence for bacterial promoters, PNAS, (1998), 95, 9761-9766.