Regulatory
Ptau

Part:BBa_K4729001

Designed by: Yasoo Morimoto   Group: iGEM23_Marburg   (2023-08-23)
Revision as of 19:36, 10 October 2023 by Yasoo mn (Talk | contribs)

Ptau - inducible promoter system


taken from:

Mostafavi, M., Lewis, J. C., Saini, T., Bustamante, J. A., Gao, I. T., Tran, T. T., King, S. N., Huang, Z., & Chen, J. C. (2014). Analysis of a taurine-dependent promoter in Sinorhizobium meliloti that offers tight modulation of gene expression. In BMC Microbiology (Vol. 14, Issue 1). Springer Science and Business Media LLC. https://doi.org/10.1186/s12866-014-0295-2

and

Seiler, V. N. (2023). Improved inducible switches for the implementation of genetic tools in Sinorhizobium meliloti and Ensifer adhaerens (Master's thesis, Philipps-University Marburg)

Measurement and comparison of different inducible promoter systems in A. rhizogenes

Figure 1: Testing different inducible promoter systems in A. rhizogenes ARqua1.

Ptau inducible promoter, induced by taurine.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 182
    Illegal PstI site found at 1411
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 1411
    Illegal NotI site found at 602
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 610
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 182
    Illegal PstI site found at 1411
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 182
    Illegal PstI site found at 1411
    Illegal NgoMIV site found at 431
    Illegal NgoMIV site found at 1219
  • 1000
    COMPATIBLE WITH RFC[1000]


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Parameters
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