Part:BBa_K4724072

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Disulfide bond formation protein A (DsbA) is an oxidoreductase enzyme present in the periplasmic space of Escherichia coli, which catalyzes the formation of disulfide bonds during the folding process of nascent proteins. It can transfer the produced proteins to the periplasmic space and catalyze the formation of disulfide bonds in nascent proteins to promote their soluble expression. The accumulation of proteins expressed in the periplasmic space can be utilized for protein purification and prevents interactions with cytoplasmic components.