RNA
Part:BBa_K4830002:Design
Designed by: Beibitkyzy Aruzhan Group: iGEM23_NTU-Singapore (2023-10-10)
RNF2 AtoC pegRNA
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The provided sequence contains the following parts: U6 promoter, spacer, guide RNA scaffold, rttpbs (A to C), polyA tail.
Source
The original RNF2 AtoC pegRNA sequence containing spacer and rttpbs was reported in Prime Editor 2 paper cited in References. We used those sequences and with the help of and TWIST Bioscience synthesized the parts, which were further pieced together with 65777 backbone in our lab.
References
Anzalone AV, Randolph PB, Davis JR, Sousa AA, Koblan LW, Levy JM, et al. Search-and-replace genome editing without double-strand breaks or donor DNA. Nature. 2019 Oct 21;576.