Composite
ML-Fok_A

Part:BBa_K243008:Design

Designed by: Freiburg Bioware09   Group: iGEM09_Freiburg_bioware   (2009-10-14)
Revision as of 14:48, 21 October 2009 by Timo (Talk | contribs) (→‎Design Notes)

Middle Linker-Fok_a


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 517


Design Notes

The middle length of the linker is useful to produce a stable connection to another part and produce certain distance. The middle distance produced by the linker reduces possible sterical interferences and increase the distance from the protein Fok_a to the DNA when it is linked to a DNA binding protein.
Designed according the assembly standard 25 RFC 25

Source

Combinated by cloning.

References