DNA

Part:BBa_K4630113:Design

Designed by: Zhejun Qin   Group: iGEM23_WHU-China   (2023-10-10)
Revision as of 00:16, 12 October 2023 by James Qin (Talk | contribs) (→‎Source)

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stgRNA-cassette (1+2)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Using Biobrick Assembly, the stgRNA-cassette 1 and stgRNA-cassette 2 can be ligated. Using the homologous arm upstream the stgRNA 2, recombination may occur between stgRNA-cassette 2 and Lac promoter of stgRNA-cassette 1.


Source

Complete synthesis and subcloning.

References