Reporter

Part:BBa_K177038

Designed by: Anna Olchowik   Group: iGEM09_Warsaw   (2009-09-09)
Revision as of 16:28, 19 October 2009 by Keton (Talk | contribs)

Bistable switch 1 testing device

Bistable switch based on termosensitive lambda repressor (cIts) and lactose operon inhibitor (lacI).


Usage and Biology

The state of the switch is set either by IPTG or temperature. In 0,1 uM IPTG lacI gets inactivated ant transcription from Plac starts. It results in production of cIts and other genes belonging to this part of switch (in this case GFP). After removal of IPTG the state remains stable - PRm is repressed by cIts and genes under Plac are expressed. In 42oC cIts gets inactivated and expression of genes under PRm (in this case lacI and RFP) are expressed. Because lacI is expressed to Plac gets repressed. The state remains stable after transfer of this device to 30oC. State of the device can be switched by pulses of IPTG and 42oC. Temperature of stable work is 30oC.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 3058
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 3645
    Illegal AgeI site found at 3757
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1614


[edit]
Categories
//function/reporter
//regulation/multiple
Parameters
chassisE. coli lacI deficient (i.e. Top10)
controlIPTG and 42oC
device_typebistable switch
functiontest device with GFP and RFP as reporters
negative_regulatorslacI and cIts
switch_pointPlac->cI lam switch in 42oC cI lam -> Plac in 0,1mM IPTG