Coding
cIts

Part:BBa_K177050:Design

Designed by: Michael Lower   Group: iGEM09_Warsaw   (2009-10-19)
Revision as of 20:43, 21 October 2009 by Keton (Talk | contribs) (→‎Source)

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cI ts


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Source

BBa_C0051 DNA was used as themplate to site directed mutagenesis. Our aim was changing T450->C to remove premature stop codon and introducion of cIts857 mutation (TTTTGCAAGCAA -> TTTTGtAAGCAA) to make this variant of cI thermosensitive.

References