Regulatory

Part:BBa_K4673026:Design

Designed by: Chieh Yu Lee   Group: iGEM23_Taipei-KCISLK-V1   (2023-10-02)
Revision as of 15:55, 9 October 2023 by KCLK 23T (Talk | contribs)

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LacI is a regulated repressor protein


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Lacl is located between the Lacl_promoter and ROP on the pEt-22b-LK vector.


Source

GSL Biotech LLC. (2023). pET-22b(+) Sequence and Map. SnapGene. https://www.snapgene.com/plasmids/pet_and_duet_vectors_(novagen)/pET-22b(%2B)

References

Lewis, M. (2005b, May 31). The lac repressor. Comptes Rendus Biologies. https://www.sciencedirect.com/science/article/pii/S1631069105000685?via%3Dihub#sec001